HI83325 is a compact, multiparameter photometer for use in the environmental lab or in the field. The meter is one of the most advanced photometers available with a innovative optical design that utilizes a reference detector and focusing lens to eliminate errors from changes in the light source and from imperfections in the glass cuvette. This meter has 9 different programmed methods measuring 8 key plant nutrient water quality parameters and also offers an absorbance measurement mode for performance verification and for users that would like to develop their own concentration versus absorbance curves. Plant nutrient-specific parameters include potassium, calcium, and magnesium.
To save valuable laboratory benchtop space, the HI83325 doubles as a professional pH meter with its digital pH/temperature electrode input. Now one meter can be used for both photometric and pH measurements.
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The HI83325 benchtop photometer measures 8 different key water quality parameters using 9 different methods. This photometer features an innovative optical system that uses LEDs, narrow band interference filters, focusing lens and both a silicon photodetector for absorbance measurement and a reference detector to maintain a consistent light source ensures accurate and repeatable photometric readings every time.
Consistent and thorough monitoring of plant nutrients is essential to maintaining healthy growth and reproduction. This is easy with the HI83325, a comprehensive way to monitor vital plant nutrients such as potassium, calcium and magnesium. Required in large quantities, potassium plays a vital role in water uptake and enzyme regulation. Calcium helps to strengthen plant cell walls protecting against heat stress while magnesium helps build a strong immune system.
A digital pH electrode input allows the user to measure pH by a traditional glass electrode. The digital pH electrode has a built in microchip within the probe that stores all of the calibration information. Having the calibration information stored in the probe allows for hot swapping of pH electrodes without having to recalibrate. All pH measurements are automatically compensated for temperature variations with a built in thermistor located in the tip of the sensing bulb for fast and accurate temperature measurement.
The HI83325 offers an absorbance measuring mode that allows for CAL Check standards to be used to validate the performance of the system. The absorbance mode allows the user to select one of the three wavelengths of light (420 nm, 466 nm, and 525 nm) to measure and plot their own concentration versus absorbance mode. This is useful for users with their own chemical method and for educators to teach the concept of absorbance by using the Beer-Lambert Law.
Two USB ports are provided for transferring data to a flash drive or computer and to use as a power source for the meter. For added convenience and portability the meter can also operate on an internal 3.7 VDC Lithium-polymer rechargeable battery.
Backlit 128 x 64 Pixel Graphic LCD Display
Built-in Reaction Timer for Photometric Measurements
Units of Measure
Digital pH Electrode Input
Battery Status Indicator
Users can easily select any one of the 20 measurement methods via the dedicated METHOD button.
Up to 1000 measurement readings can be logged with user and sample ID and recalled for future use.
pH Measurement Mode
Selecting the pH measurement mode allows for the photometer to be used as a professional pH meter with many features including temperature compensated measurements, automatic two point calibration, and GLP.
HI83325 is designed with an innovative optical system that incorporates a beam splitter so that light can be used for absorbance readings and for a reference detector. The reference detector monitors the intensity of light and modulates when there is drift due to power fluctuation or the heating of the optical components. Each part has an important role in providing unparalleled performance from a photometer.
An LED light source offers superior performance as compared to a tungsten lamp. LEDs have a much higher luminous efficiency, providing more light while using less power. They also produce very little heat, which could otherwise affect the optical components an electronic stability. LEDs are available in a wide array of wavelengths, whereas tungsten lamps are supposed to be white light (all wavelengths of visible light) but actually have a poor blue/violet light output.
The narrow band interference filter not only ensure greater wavelength accuracy (+/- 1 nm) but are extremely efficient. The filters used allow up to 95% of the light from the LED to be transmitted as compared to other filters that are only 75% efficient. The higher efficiency allows for a brighter, stronger light source. The end result is higher measurement stability and less wavelength error.
A beam splitter is used as part of the internal reference system of the HI83326 photometer. The reference detector compensates for any drift due to power fluctuations or ambient temperature changes. Now you can rely on a stable source of light between your blank (zero) measurement and sample measurement.
The sample cell of the HI83326 fits a round, glass cuvette with a 25 mm path length. Along with the advanced optical components, the larger size of the cuvette greatly reduces errors in rotation from the indexing mark of the cuvettes. The relatively long path length of the sample cuvette allows the light to pass through more of the sample solution, ensuring accurate measurements even in low absorbance samples.
Adding a focusing lens to the optical path allows for the collection of all of the light that exits the cuvette and focusing the light on the silicon photo detector. This novel approach to photometric measurements cancels the errors from imperfections and scratches present in the glass cuvette eliminating the need to index the cuvette.
Photometer: 6.5 to 8.5 pH
pH electrode: -2.00 to 16.00 pH
Photometer: 0.1 pH
pH electrode: 0.01 pH
Photometer: ±0.1 pH
pH electrode: ±0.01 pH
Automatic one or two point calibration with one set of standard buffers available (4.01, 6.86, 7.01, 9.18, 10.01)
Automatic (-5.0 to 100.0 oC; 23.0 to 212.0 oF); limits reduced based on the pH electrode used
Cal Check / Electrode diagnostic
clean electrode and check buffer/check probe displayed during calibration
Photometer: phenol red
0.000 to 4.000 Abs
±0.003Abs @ 1.000 Abs
Low Range: 0.00 to 3.00 mg/L
Medium Range: 0.00 to 10.00 mg/L
High Range: 0.0 to 100.0 mg/L
0.01 mg/L; 0.1 mg/L
Low Range: ±0.04 mg/L ±4% of reading
Medium Range: ±0.05 mg/L ±5% of reading
High range: ±0.5 mg/L ±5% of reading
Adaptation of the ASTM Manual of Water and Environmental Technology, D1426-92, Nessler method
Freshwater: 0 to 400 mg/L (as Ca2+)
Freshwater: ±10 mg/L ±5% of reading
Freshwater: Adaptation of the Oxalate method
0 to 150 mg/L (as Mg2+)
±5 mg/L ±3% of reading
Adaptation of the Calmagite method
0.0 to 30.0 mg/L (as NO3–– N)
±0.5 mg/L ±10% of reading
Adaptation of the cadmium reduction method
High range: 0.0 to 30.0 mg/L(as PO43-)
±1 mg/L ±4% of reading
Adaptation of the Standard Methods for the Examination of Water and Wastewater, 18th edition, Amino Acid method
0.0 to 20.0 mg/L (as K)
±3.0 mg/L ±7% of reading
Adaptation of the Turbidimetric Tetraphenylborate method
0 to 150 mg/L (as SO42-)
±5 mg/L ±3% of reading
Turbidimetric – Sulfate is precipitated with barium chloride crystals
1 pH electrode input and 5 photometer wavelengths
digital pH electrode (not included)
og on demand with user name and sample ID optional input
USB-A host for flash drive; micro-USB-B for power and computer connectivity
calibration data for connected pH electrode
128 x 64 pixel LCD with backlight
3.7 VDC Li-polymer rechargeable battery / >500 photometric measurements or 50 hours of continuous pH measurement
5 VDC USB 2.0 power adapter with USB-A to micro-USB-B cable (included)
0 to 50.0 oC (32 to 122.0 oF); 0 to 95% RH, non-condensing
206 x 177 x 97 mm (8.1 x 7.0 x 3.8″)
1.0 kg (2.2 lbs.)
Photometer/Colorimeter Light Source
5 LEDs with 420 nm, 466 nm, 525 nm, 575 nm, and 610 nm narrow band interference filters
Bandpass Filter Bandwidth
Bandpass Filter Wavelength Accuracy
round, 24.6 mm
Number of Methods
HI83225 is supplied with sample cuvettes and caps (4 ea.), cloth for wiping cuvettes, USB to micro USB cable connector, power adapter and instruction manual.